This product is a recombinant bacteriophage T7 RNA polymerase expressed in E. coli. It utilizes double-stranded DNA containing the T7 promoter sequence (5′-TAATACGACTCACTATAG*-3′) as a template and NTPs as substrates to synthesize RNA complementary to the antisense strand downstream of the promoter. The enzyme can utilize both blunt-ended and 5′-overhang linear double-stranded DNA as templates, making linearized plasmids or PCR products suitable for in vitro RNA synthesis.
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Gene Name |
T7 RNA polymerase |
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Expression host |
E. coli |
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Source |
Escherichia phage T7 (Bacteriophage T7) |
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Tag |
His tag |
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Molecular Weight |
98.9 kDa |
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Enzyme Activity |
20 U/μL |
|
Purity |
≥90 % by SDS-PAGE under reducing conditions |
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Storage Buffer |
50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50%（v/v）glycerol ，pH7.9@25℃ |
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Unit Definition |
Under conditions of 37°C and pH 8.0, the amount of enzyme required to incorporate 1 nmol of [³H]GMP into acid-insoluble precipitate within 1 hour is defined as 1 unit of activity (U). |
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Shipping |
The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below. |
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Storage |
The product should be stored at -20℃ . Avoid repeated freeze-thaw cycles. |
